Transmission electron microscopes are capable of imaging at a significantly higher resolution than light microscopes, owing to the smaller de Broglie wavelength of electrons. The image is then magnified and focused onto an imaging device, such as a fluorescent screen, a layer of photographic film, or a sensor such as a scintillator attached to a charge-coupled device. An image is formed from the interaction of the electrons with the sample as the beam is transmitted through the specimen. The specimen is most often an ultrathin section less than 100 nm thick or a suspension on a grid. Transmission electron microscopy ( TEM) is a microscopy technique in which a beam of electrons is transmitted through a specimen to form an image. ![]() Operating principle of a transmission electron microscope Technique in microscopy A TEM image of a cluster of poliovirus.
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